4WFT
Crystal structure of tRNA-dihydrouridine(20) synthase dsRBD domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-17 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 |
Unit cell lengths | 55.130, 55.130, 114.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.738 - 1.700 |
R-factor | 0.1639 |
Rwork | 0.163 |
R-free | 0.18020 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.047 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.740 | 1.750 |
High resolution limit [Å] | 1.700 | 1.690 |
Rmerge | 0.336 | |
Number of reflections | 38156 | |
<I/σ(I)> | 22.06 | 4.16 |
Completeness [%] | 99.7 | 97.65 |
Redundancy | 4.51 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | Protein 20 mg/mL precipitant: HEPES 100 mM pH 6.5, sodium acetate 100 mM, PEG 2000 MME 30% | |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | Protein 20 mg/mL precipitant: HEPES 100 mM pH 6.5, sodium acetate 100 mM, PEG 2000 MME 30% |