4WFS
Crystal Structure of tRNA-dihydrouridine(20) synthase catalytic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-16 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9801 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 71.570, 85.260, 144.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.640 - 2.680 |
R-factor | 0.1985 |
Rwork | 0.197 |
R-free | 0.22170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b0p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.640 | 2.770 |
High resolution limit [Å] | 2.680 | 2.680 |
Number of reflections | 12686 | |
<I/σ(I)> | 19.87 | 2.69 |
Completeness [%] | 99.1 | 91.76 |
Redundancy | 7.2 | 7.12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | Protein 8 mg/mL in Tris 25 mM pH 8.0 NaCl 150 mM Precipitant: Ammonium sulfate 2.2 M, isopropanol 2% v/v |