4WFQ

Crystal structure of TFIIH subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 32 2 1
Unit cell lengths	78.594, 78.594, 83.903
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.680 - 2.400
R-factor	0.1955
Rwork	0.195
R-free	0.21360
RMSD bond length	0.008
RMSD bond angle	1.170
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	20596
<I/σ(I)>	27.4
Completeness [%]	100.0
Redundancy	20.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG