4WFM
Structure of the complete bacterial SRP Alu domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 176.254, 194.345, 82.992 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.548 - 3.100 |
| R-factor | 0.1922 |
| Rwork | 0.191 |
| R-free | 0.20750 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.524 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.050 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 26252 | |
| <I/σ(I)> | 13.8 | 2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | sodium cacodylate, MgCl2, sodium formate, LiSO4 |
