4WFB
The crystal structure of the large ribosomal subunit of Staphylococcus aureus in complex with BC-3205
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.873 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 280.918, 280.918, 875.585 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.539 - 3.430 |
R-factor | 0.2061 |
Rwork | 0.204 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wce |
RMSD bond length | 0.006 |
RMSD bond angle | 1.099 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.470 |
High resolution limit [Å] | 3.430 | 10.620 | 3.430 |
Rmerge | 0.152 | 0.098 | 0.744 |
Total number of observations | 2109298 | ||
Number of reflections | 253918 | ||
<I/σ(I)> | 6 | ||
Completeness [%] | 92.1 | 99.7 | 78.9 |
Redundancy | 8.3 | 15.9 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Hepes, KCl, MgCl2, NH4Cl, MPD, EtOH, Spermidine |