4WFB
The crystal structure of the large ribosomal subunit of Staphylococcus aureus in complex with BC-3205
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 280.918, 280.918, 875.585 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.539 - 3.430 |
| R-factor | 0.2061 |
| Rwork | 0.204 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wce |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.099 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.470 |
| High resolution limit [Å] | 3.430 | 10.620 | 3.430 |
| Rmerge | 0.152 | 0.098 | 0.744 |
| Total number of observations | 2109298 | ||
| Number of reflections | 253918 | ||
| <I/σ(I)> | 6 | ||
| Completeness [%] | 92.1 | 99.7 | 78.9 |
| Redundancy | 8.3 | 15.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Hepes, KCl, MgCl2, NH4Cl, MPD, EtOH, Spermidine |
