4WFA

The crystal structure of the large ribosomal subunit of Staphylococcus aureus in complex with linezolid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.873,0.972,1.000
Spacegroup name	P 65 2 2
Unit cell lengths	279.922, 279.922, 870.586
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.877 - 3.392
R-factor	0.2038
Rwork	0.202
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wce
RMSD bond length	0.006
RMSD bond angle	1.086
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	200.000	200.000	3.460
High resolution limit [Å]	3.400	9.230	3.400
Rmerge	0.156	0.070	0.776
Rmeas	0.170	0.077	0.895
Rpim	0.061	0.029	0.419
Total number of observations	1398748
Number of reflections	246474
<I/σ(I)>	5.7
Completeness [%]	89.4	88.2	64.8
Redundancy	5.7	5.6	2.8
CC(1/2)		0.996	0.433

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Hepes, KCl, MgCl2, NH4Cl, MPD, EtOH, Spermidine