4WEI
Crystal structure of the F4 fimbrial adhesin FaeG in complex with lactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 93.194, 93.194, 110.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.670 - 2.300 |
R-factor | 0.19438 |
Rwork | 0.192 |
R-free | 0.23029 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hlr |
RMSD bond length | 0.018 |
RMSD bond angle | 1.942 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.670 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 22359 | |
<I/σ(I)> | 19.9 | 2.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.7 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Trizma/Bicine pH 8.5, 0.03 M of each halogen (sodium fluoride, sodium bromide, sodium iodide), 12.5% MPD, 12.5% PEG1000, 12.5% PEG3350 |