4WEI
Crystal structure of the F4 fimbrial adhesin FaeG in complex with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.194, 93.194, 110.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.670 - 2.300 |
| R-factor | 0.19438 |
| Rwork | 0.192 |
| R-free | 0.23029 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hlr |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.942 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.670 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 22359 | |
| <I/σ(I)> | 19.9 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.7 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Trizma/Bicine pH 8.5, 0.03 M of each halogen (sodium fluoride, sodium bromide, sodium iodide), 12.5% MPD, 12.5% PEG1000, 12.5% PEG3350 |
