Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.960, 194.570, 37.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.690 - 2.400 |
| R-factor | 0.2038 |
| Rwork | 0.199 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.358 |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 24.690 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 24344 |
| <I/σ(I)> | 9.6 |
| Completeness [%] | 100.0 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 22% (w/v) PEG 3350, 0.5 M MgCl2, 0.1 M HEPES, 15% dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
