4WE7
Structure and receptor binding preferences of recombinant human A(H3N2) virus hemagglutinins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-10-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.298, 214.898, 101.807 |
| Unit cell angles | 90.00, 103.58, 90.00 |
Refinement procedure
| Resolution | 42.980 - 2.500 |
| R-factor | 0.2202 |
| Rwork | 0.218 |
| R-free | 0.25110 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.264 |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 67875 |
| <I/σ(I)> | 8.7 |
| Completeness [%] | 98.1 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6 | 293 | 0.2M Calcium acetate, 0.1M MES, 20% PEG 8000 |
