4WE6

The crystal structure of hemagglutinin HA1 domain from influenza virus A/Perth/142/2007(H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-02-27
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.0
Spacegroup name	P 1 2 1
Unit cell lengths	42.472, 50.567, 182.256
Unit cell angles	90.00, 90.08, 90.00

Refinement procedure

Resolution	44.216 - 1.904
R-factor	0.1711
Rwork	0.170
R-free	0.20110
RMSD bond length	0.020
RMSD bond angle	1.683
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	56707
<I/σ(I)>	12.8
Completeness [%]	98.2
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	8.5	293	0.1M MgCl2, 0.1 Tris-HCl, pH8.5, 25% PEG 3350