4WE4

The crystal structure of hemagglutinin from 1968 H3N2 influenza virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	I 21 3
Unit cell lengths	155.506, 155.506, 155.506
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.497 - 2.351
R-factor	0.1954
Rwork	0.193
R-free	0.23600
RMSD bond length	0.005
RMSD bond angle	0.923
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.350
Number of reflections	25624
<I/σ(I)>	40
Completeness [%]	98.1
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.9	293	0.1M Tris-HCl, 25% PEG1000