4WDC
High-resolution crystal structure of water-soluble FraC (mutation F16P)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.830, 61.480, 66.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.100 - 1.290 |
| R-factor | 0.12005 |
| Rwork | 0.119 |
| R-free | 0.15144 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.629 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.100 | 1.360 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmerge | 0.065 | 0.540 |
| Number of reflections | 42888 | |
| <I/σ(I)> | 16.6 | 2.6 |
| Completeness [%] | 95.2 | 73.1 |
| Redundancy | 6.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 22% PEG3500, 200mM Potasium fluoride |
