4WD9
Crystal structure of tRNA-dependent lantibiotic dehydratase NisB in complex with NisA leader peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-03-09 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.500, 107.280, 135.730 |
Unit cell angles | 90.00, 109.92, 90.00 |
Refinement procedure
Resolution | 34.459 - 2.900 |
R-factor | 0.1974 |
Rwork | 0.194 |
R-free | 0.25690 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.361 |
Data reduction software | XDS |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.500 |
High resolution limit [Å] | 2.900 |
Number of reflections | 58894 |
<I/σ(I)> | 10.6 |
Completeness [%] | 99.6 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 282 | 100mM Bicine pH 8.5, 15%-25% PEG 6000 |