4WD7
Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae by Zn-SAD phasing
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-26 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 1.2821 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 114.124, 160.587, 161.745 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.189 - 2.900 |
R-factor | 0.2399 |
Rwork | 0.239 |
R-free | 0.25280 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.881 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 66573 |
<I/σ(I)> | 29.1 |
Completeness [%] | 100.0 |
Redundancy | 29.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.05 M zinc acetate, 6% v/v ethylene glycol, 0.1 M sodium cacodylate, pH 6.0, 6.6 % w/v PEG 8000 |