4WD6
Crystal Structure of DIM-1 metallo-beta-lactamase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.719, 47.856, 185.216 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.550 - 2.200 |
| R-factor | 0.20989 |
| Rwork | 0.207 |
| R-free | 0.27397 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ddk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.617 |
| Data reduction software | HKL-2000 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.051 | 0.251 |
| Number of reflections | 21133 | |
| <I/σ(I)> | 32.5 | 6.6 |
| Completeness [%] | 94.5 | 68.4 |
| Redundancy | 7.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% polyethylene glycol (PEG) 550 monomethyl ether (MME), 10% PEG 20000, 50 mM HEPES / 50 mM MOPS pH 7.5 |
