4WCX
Crystal structure of HydG: A maturase of the [FeFe]-hydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9763, 1.7389 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.130, 56.190, 84.920 |
| Unit cell angles | 89.59, 83.62, 66.84 |
Refinement procedure
| Resolution | 84.327 - 1.590 |
| R-factor | 0.1805 |
| Rwork | 0.180 |
| R-free | 0.21340 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.866 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.330 | 1.630 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.039 | 0.349 |
| Number of reflections | 118163 | |
| <I/σ(I)> | 12.3 | 2.2 |
| Completeness [%] | 96.2 | 94.5 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM Bis-TRIS propane, 200 mM sodium fluoride, 20% polyethylene glycol 3,350 |
