4WCW
Ribosomal silencing factor during starvation or stationary phase (RsfS) from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2014-02-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 50.975, 50.975, 64.546 |
Unit cell angles | 110.27, 96.17, 110.59 |
Refinement procedure
Resolution | 46.060 - 2.100 |
R-factor | 0.2127 |
Rwork | 0.210 |
R-free | 0.25560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o5a |
RMSD bond length | 0.006 |
RMSD bond angle | 0.892 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.060 |
High resolution limit [Å] | 2.100 |
Number of reflections | 49832 |
<I/σ(I)> | 6.3 |
Completeness [%] | 93.3 |
Redundancy | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M Sodium Cacodylate pH6.5, 0.2 M Magnesium Acetate, 30% MPD |