4WCW

Ribosomal silencing factor during starvation or stationary phase (RsfS) from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	120
Detector technology	CCD
Collection date	2014-02-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	50.975, 50.975, 64.546
Unit cell angles	110.27, 96.17, 110.59

Refinement procedure

Resolution	46.060 - 2.100
R-factor	0.2127
Rwork	0.210
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o5a
RMSD bond length	0.006
RMSD bond angle	0.892
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.060
High resolution limit [Å]	2.100
Number of reflections	49832
<I/σ(I)>	6.3
Completeness [%]	93.3
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M Sodium Cacodylate pH6.5, 0.2 M Magnesium Acetate, 30% MPD