4WCL
Crystal Structure of product bound Cell Shape Determinant protein Csd4 from Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-12 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.030, 66.770, 144.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.758 - 1.850 |
R-factor | 0.1709 |
Rwork | 0.170 |
R-free | 0.19530 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.089 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.760 | 1.900 | |
High resolution limit [Å] | 1.850 | 8.270 | 1.850 |
Rmerge | 0.053 | 0.021 | 0.388 |
Rmeas | 0.062 | 0.024 | 0.455 |
Total number of observations | 315590 | ||
Number of reflections | 84317 | 904 | 6254 |
<I/σ(I)> | 15.53 | 42.02 | 3.13 |
Completeness [%] | 99.6 | 95.4 | 99.2 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 16-20% PEG 3350, 0-0.1 mM Tris and 0.3-0.4 M NaI |