4WC7

Structure of tRNA-processing enzyme complex 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2014-06-18
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	58.870, 111.390, 127.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.835 - 3.102
R-factor	0.2794
Rwork	0.277
R-free	0.32180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wc2
RMSD bond length	0.003
RMSD bond angle	0.580
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		3.180
High resolution limit [Å]	3.100	13.870	3.100
Rmerge	0.137	0.035	1.590
Rmeas	0.149	0.039	1.727
Total number of observations	101099
Number of reflections	15717	125	1104
<I/σ(I)>	11.61	37.79	1.23
Completeness [%]	99.2	56.6	98.5
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	isopropanol, potassium chloride, magnesium chloride, sodium cacodylate