4WC6

Structure of tRNA-processing enzyme complex 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2014-06-18
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	59.220, 111.740, 127.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.872 - 3.410
R-factor	0.27
Rwork	0.267
R-free	0.32730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wc2
RMSD bond length	0.002
RMSD bond angle	0.507
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		3.490
High resolution limit [Å]	3.410	15.230	3.410
Rmerge	0.207	0.051	2.056
Rmeas	0.215	0.054	2.131
Total number of observations	172906
Number of reflections	12086	89	883
<I/σ(I)>	11.84	36.3	1.77
Completeness [%]	99.2	51.1	99.9
Redundancy	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	isopropanol, potassium chloride, magnesium chloride, sodium cacodylate