4WC3
Structure of tRNA-processing enzyme complex 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-10-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.660, 110.300, 126.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.841 - 3.100 |
| R-factor | 0.2429 |
| Rwork | 0.241 |
| R-free | 0.28850 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.621 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.180 | |
| High resolution limit [Å] | 3.100 | 13.860 | 3.100 |
| Rmerge | 0.124 | 0.038 | 3.123 |
| Rmeas | 0.129 | 0.039 | 3.243 |
| Total number of observations | 215413 | ||
| Number of reflections | 15234 | 119 | 1068 |
| <I/σ(I)> | 17.33 | 54.84 | 1.07 |
| Completeness [%] | 98.4 | 55.6 | 97.3 |
| Redundancy | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | isopropanol, potassium chloride, magnesium chloride, sodium cacodylate |
