4WC2

Crystal structure of tRNA nucleotidyltransferase complexed with a primer tRNA and an incoming ATP analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-12-09
Detector	RIGAKU RAXIS V
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	58.920, 109.480, 125.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.802 - 2.800
R-factor	0.218
Rwork	0.215
R-free	0.26720
RMSD bond length	0.002
RMSD bond angle	0.615
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.067	0.398
Number of reflections	20480
<I/σ(I)>	22.4	4.3
Completeness [%]	99.2	95.7
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	MPD, magnesium acetate