4WC1

Structure of tRNA-processing enzyme with CTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2013-01-25
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97897
Spacegroup name	P 1 21 1
Unit cell lengths	58.400, 108.090, 84.920
Unit cell angles	90.00, 110.08, 90.00

Refinement procedure

Resolution	19.833 - 3.100
R-factor	0.1846
Rwork	0.181
R-free	0.23090
Starting model (for MR)	4wby
RMSD bond length	0.005
RMSD bond angle	0.925
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		3.180
High resolution limit [Å]	3.100	13.850	3.100
Rmerge	0.076	0.024	1.267
Rmeas	0.088	0.030	1.476
Total number of observations	67902
Number of reflections	17858	118	1306
<I/σ(I)>	16.08	47.58	1.19
Completeness [%]	98.2	53.4	97.5
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEGMME550, sodium chloride, Tris-Cl