4WC1
Structure of tRNA-processing enzyme with CTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-01-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97897 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.400, 108.090, 84.920 |
Unit cell angles | 90.00, 110.08, 90.00 |
Refinement procedure
Resolution | 19.833 - 3.100 |
R-factor | 0.1846 |
Rwork | 0.181 |
R-free | 0.23090 |
Starting model (for MR) | 4wby |
RMSD bond length | 0.005 |
RMSD bond angle | 0.925 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.180 | |
High resolution limit [Å] | 3.100 | 13.850 | 3.100 |
Rmerge | 0.076 | 0.024 | 1.267 |
Rmeas | 0.088 | 0.030 | 1.476 |
Total number of observations | 67902 | ||
Number of reflections | 17858 | 118 | 1306 |
<I/σ(I)> | 16.08 | 47.58 | 1.19 |
Completeness [%] | 98.2 | 53.4 | 97.5 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEGMME550, sodium chloride, Tris-Cl |