4WBZ
tRNA-processing enzyme (apo form 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2012-12-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.600, 109.660, 85.270 |
Unit cell angles | 90.00, 110.09, 90.00 |
Refinement procedure
Resolution | 19.867 - 3.300 |
R-factor | 0.2036 |
Rwork | 0.203 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wby |
RMSD bond length | 0.002 |
RMSD bond angle | 0.611 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.390 | |
High resolution limit [Å] | 3.300 | 14.760 | 3.300 |
Rmerge | 0.170 | 0.026 | 1.558 |
Rmeas | 0.195 | 0.032 | 1.790 |
Total number of observations | 64039 | ||
Number of reflections | 15192 | 158 | 1036 |
<I/σ(I)> | 9.27 | 40.07 | 1.07 |
Completeness [%] | 99.0 | 88.3 | 92 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEGMME550, NaCl, Tris-Cl |