4WBY

tRNA-processing enzyme (apo form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2010-05-18
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	54.510, 67.740, 127.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.940 - 1.500
R-factor	0.1568
Rwork	0.156
R-free	0.17610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vfg
RMSD bond length	0.005
RMSD bond angle	0.958
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		1.540
High resolution limit [Å]	1.500	6.710	1.500
Rmerge	0.040	0.031	0.382
Rmeas	0.043	0.034	0.404
Total number of observations	719782
Number of reflections	75966	752	5526
<I/σ(I)>	30.42	54.59	6.13
Completeness [%]	99.5	77.6	99.4
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG3350, potassium sulfate