4WBQ
Crystal structure of the exonuclease domain of QIP (QDE-2 interacting protein) solved by native-SAD phasing.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-08 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 2.0664 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 77.040, 77.040, 112.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.963 - 2.693 |
| R-factor | 0.1748 |
| Rwork | 0.172 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.587 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.260 |
| High resolution limit [Å] | 2.693 | 2.200 |
| Rmerge | 0.134 | |
| Number of reflections | 20707 | |
| <I/σ(I)> | 37.1 | 16.94 |
| Completeness [%] | 99.9 | 86.8 |
| Redundancy | 154 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1M CaCl2, 0.1M Hepes buffer, pH7, 20% PEG6000 |
