4WBP
Crystal structure of the HR-1 domain of human caprin-1 in the P3121 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-22 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97872 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 66.000, 66.000, 117.701 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.230 - 2.500 |
R-factor | 0.2177 |
Rwork | 0.212 |
R-free | 0.26560 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.914 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.230 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 10750 | |
<I/σ(I)> | 12.3 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.6 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 12% PEG600, 0.1 M Tris, 0.1 M Calcium acetate, 10% glycerol |