4WBE
Crystal structure of the HR-1 domain of human caprin-1 in the C121 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.950, 77.460, 58.020 |
| Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
| Resolution | 32.384 - 2.050 |
| R-factor | 0.1986 |
| Rwork | 0.196 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.942 |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.380 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 29462 |
| <I/σ(I)> | 5.5 |
| Completeness [%] | 98.9 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 22% PEG750MME, 0.1 M Tris, 0.1 M potassium fluoride, 10% glycerol |
