4WBA
Q/E mutant SA11 NSP4_CCD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9774 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.857, 53.469, 52.543 |
Unit cell angles | 90.00, 95.84, 90.00 |
Refinement procedure
Resolution | 28.240 - 1.799 |
R-factor | 0.1899 |
Rwork | 0.187 |
R-free | 0.23690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o1k |
RMSD bond length | 0.006 |
RMSD bond angle | 0.923 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.240 |
High resolution limit [Å] | 1.799 |
Rmerge | 0.060 |
Number of reflections | 20053 |
<I/σ(I)> | 15.21 |
Completeness [%] | 99.8 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 296 | 10%w/v PEG 8000, 0.2M NaCl, 0.1M Na/K PO4 (pH 6.2) |