4WB9
Human ALDH1A1 complexed with NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 4 2 2 |
| Unit cell lengths | 109.415, 109.415, 83.448 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 109.420 - 2.070 |
| R-factor | 0.17689 |
| Rwork | 0.174 |
| R-free | 0.23387 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.767 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.420 | 2.110 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.091 | 0.520 |
| <I/σ(I)> | 17.8 | 4.9 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 8.3 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 300 | PEG3350, sodium chloride, 100mM BisTris Buffer, ytterbium chloride, NAD, sitting drop |
