4WB0
Crystal structure of the broad specificity aminotransferase from Leishmania mexicana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.418, 90.564, 142.159 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.381 - 1.910 |
| R-factor | 0.1783 |
| Rwork | 0.177 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cst |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.415 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.400 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.055 | 0.629 |
| Number of reflections | 86628 | |
| <I/σ(I)> | 17.4 | 2.1 |
| Completeness [%] | 99.3 | 95.8 |
| Redundancy | 4.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 284 | 1 M tri-sodium citrate, 0.1 M sodium cacodylate |
