4WAN
Crystal structure of Msl5 protein in complex with RNA at 1.8 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.380, 130.970, 53.370 |
| Unit cell angles | 90.00, 117.43, 90.00 |
Refinement procedure
| Resolution | 45.610 - 1.800 |
| R-factor | 0.18 |
| Rwork | 0.175 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.065 |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.610 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 | 0.357 |
| Number of reflections | 59297 | |
| <I/σ(I)> | 9.5 | 3.4 |
| Completeness [%] | 99.3 | 95.4 |
| Redundancy | 5.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 2.1-2.3 M ammonium sulfate, 0.01 M magnesium acetate, 0.05 M MES |
