4WAI

Structural characterization of the late competence protein ComFB from Bacillus subtilis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-10
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	41.483, 123.579, 41.759
Unit cell angles	90.00, 93.92, 90.00

Refinement procedure

Resolution	34.540 - 2.427
R-factor	0.2122
Rwork	0.208
R-free	0.25090
RMSD bond length	0.014
RMSD bond angle	1.181
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.543	2.513
High resolution limit [Å]	2.427	2.427
Rmerge	0.101
Number of reflections	14767
<I/σ(I)>	6.18	1.39
Completeness [%]	93.0
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298.15	22 % PEG 3350, 0.2 M NaCl, 0.1 M BisTris