4WAD
Crystal Structure of TarM with UDP-GlcNAc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-01 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 124.024, 124.024, 217.030 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.132 - 2.800 |
R-factor | 0.2539 |
Rwork | 0.252 |
R-free | 0.29390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wac |
RMSD bond length | 0.004 |
RMSD bond angle | 0.799 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.870 | |
High resolution limit [Å] | 2.800 | 12.520 | 2.800 |
Rmerge | 0.234 | 0.037 | 2.263 |
Rmeas | 0.243 | 0.039 | 2.355 |
Total number of observations | 326267 | ||
Number of reflections | 24904 | 340 | 1822 |
<I/σ(I)> | 11.69 | 45.24 | 1.27 |
Completeness [%] | 99.3 | 93.9 | 100 |
Redundancy | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | Magnesium-chloride, PEG-8000 |