4WAA
Crystal structure of Nix LIR-fused human LC3B_2-119
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.385, 67.385, 116.875 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.150 - 2.350 |
| R-factor | 0.20311 |
| Rwork | 0.200 |
| R-free | 0.27283 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vtu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.558 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.990 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.157 | 0.567 |
| Number of reflections | 11860 | |
| <I/σ(I)> | 15.7 | 5.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 300 | 20% PEG3350, 8% Tacsimate pH 4 |
