4WA1
The crystal structure of hemagglutinin from a H3N8 influenza virus isolated from New England harbor seals
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 80.061, 103.155, 110.864 |
Unit cell angles | 89.95, 90.00, 90.28 |
Refinement procedure
Resolution | 46.779 - 1.898 |
R-factor | 0.1709 |
Rwork | 0.170 |
R-free | 0.19490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yp2 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.134 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.898 |
Number of reflections | 272212 |
<I/σ(I)> | 12.4 |
Completeness [%] | 97.1 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 293 | 0.2 M ammonium iodide, 20% PEG3350 |