4WA0
The structure of a possible adhesin C-terminal domain from Caldicellulosiruptor kronotskyensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-24 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.507, 75.290, 77.446 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.980 - 1.700 |
| R-factor | 0.1522 |
| Rwork | 0.150 |
| R-free | 0.19410 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.969 |
| Data scaling software | PROTEUM PLUS (3013.8-1) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 40244 | |
| <I/σ(I)> | 17.48 | 2.36 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 8.24 | 5.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.4 | 294 | PEG ion HT screen from Hampton Research (Aliso Viejo, CA) condition G12 containing 0.07 M Citric acid, 0.03 M BIS-TRIS propane, and 16% (w/v) Polyethylene glycol 3350 |
