4W9Z
Crystal structure of the periplasmic domain of subunit II of cytochrome oxidase (CoxB) of Bradyrhizobium japonicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2014-08-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.431, 38.612, 59.644 |
Unit cell angles | 90.00, 97.63, 90.00 |
Refinement procedure
Resolution | 59.120 - 1.300 |
R-factor | 0.1102 |
Rwork | 0.109 |
R-free | 0.13360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4txv |
RMSD bond length | 0.026 |
RMSD bond angle | 2.270 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 1.330 | ||
High resolution limit [Å] | 1.300 | 5.810 | 1.300 |
Rmerge | 0.048 | 0.033 | 0.247 |
Rmeas | 0.053 | 0.035 | 0.272 |
Total number of observations | 231266 | ||
Number of reflections | 35883 | 437 | 2652 |
<I/σ(I)> | 20.13 | 53.23 | 5.25 |
Completeness [%] | 99.2 | 98.9 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2M Potassium bromide, 0.1M Tris pH 7.5, 10% PEG1000, 10% PEG8000 |