4W9B
Crystal structure of Gamma-B Crystallin expressed in E. coli based on mRNA variant 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | H 3 2 |
Unit cell lengths | 123.641, 123.641, 56.548 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.292 - 1.279 |
R-factor | 0.1706 |
Rwork | 0.170 |
R-free | 0.18760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gcr |
RMSD bond length | 0.005 |
RMSD bond angle | 1.028 |
Data reduction software | XDS (XDSapp) |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.880 | 1.360 |
High resolution limit [Å] | 1.279 | 1.279 |
Number of reflections | 42474 | |
<I/σ(I)> | 19 | 2.27 |
Completeness [%] | 99.7 | 99.3 |
Redundancy | 11.14 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 1% Trypton, 20% PEG 3350, 50mM HEPES |