4W9A
Crystal structure of Gamma-B Crystallin expressed in E. coli based on mRNA variant 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 123.644, 123.644, 56.568 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.295 - 1.380 |
| R-factor | 0.1644 |
| Rwork | 0.163 |
| R-free | 0.18440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gcr |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.018 |
| Data reduction software | XDS (XDSapp) |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.890 | 1.460 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Number of reflections | 33993 | |
| <I/σ(I)> | 19.93 | 2.16 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 5.93 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 1% Trypton, 20% PEG 3350, 50mM HEPES |
