4W92
Crystal structure of Bacillus subtilis cyclic-di-AMP riboswitch ydaO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 60.281, 83.062, 233.573 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.787 - 3.209 |
R-factor | 0.2553 |
Rwork | 0.252 |
R-free | 0.28920 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.728 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
High resolution limit [Å] | 3.200 | 8.670 | 3.200 |
Rmerge | 0.166 | 0.059 | |
Rmeas | 0.165 | 0.062 | |
Rpim | 0.056 | 0.018 | 0.491 |
Total number of observations | 122045 | ||
Number of reflections | 9964 | ||
<I/σ(I)> | 5.1 | ||
Completeness [%] | 99.2 | 99.3 | 91.1 |
Redundancy | 12.2 | 12.2 | 6.8 |
CC(1/2) | 1.000 | 0.513 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | PEG 3350 |