4W8X
Crystal Structure of Cmr1 from Pyrococcus furiosus bound to a nucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.057, 101.057, 107.947 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.518 - 3.000 |
| R-factor | 0.1716 |
| Rwork | 0.170 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w8z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.215 |
| Data reduction software | XDS |
| Refinement software | PHENIX ((phenix.refine: 1.9_1678)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.500 | 3.070 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Number of reflections | 25373 | |
| <I/σ(I)> | 15.18 | 1.6 |
| Completeness [%] | 94.9 | 89.2 |
| Redundancy | 9.4 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 100 mM bicine/Trizma base pH 6.0, 10% PEG 8000, 20% ethylene glycol, 20 mM each of sodium DL- alanine, sodium L-gutamate, gylcine, DL-lysine HCl, DL-serine, 3% DMSO |
