4W8W

Crystal structure of oligomeric Cmr4 from Pyrococcus furiosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	63.707, 129.509, 195.943
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.375 - 2.803
R-factor	0.1982
Rwork	0.197
R-free	0.23000
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.625
Data reduction software	XDS
Refinement software	PHENIX ((phenix.refine: 1.9_1678))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.380	2.903
High resolution limit [Å]	2.800	2.803
Number of reflections	76539
<I/σ(I)>	12.61	1.22
Completeness [%]	99.9	98.8
Redundancy	6.5	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	30% Jeffamine M-600 and 100 mM HEPES pH 7.0