4W8C

Crystal structure of the helical domain deleted form MsrA from Clostridium oremlandii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	C 2 2 2
Unit cell lengths	65.851, 94.475, 122.647
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.600 - 1.757
R-factor	0.2288
Rwork	0.226
R-free	0.27520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lwj
RMSD bond length	0.016
RMSD bond angle	1.613
Data reduction software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.700
Number of reflections	36941
<I/σ(I)>	49.7
Completeness [%]	96.4
Redundancy	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris-HCl, 3.2 M NaCl