4W80
Tetrameric BAP29 vDED with disulfide bonds in crystal contacts
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 117.910, 117.910, 168.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.687 - 3.200 |
| R-factor | 0.1993 |
| Rwork | 0.199 |
| R-free | 0.21520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w7z |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.789 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.690 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 9752 | |
| <I/σ(I)> | 12.21 | |
| Completeness [%] | 96.1 | 98.2 |
| Redundancy | 13.4 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 277.15 | Grown in 1.8 M ammonium sulfate, 100 mM Na acetate pH 4.6 using protein purified in 20 mM Tris pH 8, 150 mM NaCl and 0.5 mM TCEP. |
