4W7H
Crystal Structure of DEH Reductase A1-R Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 104.500, 104.500, 68.116 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.110 |
| R-factor | 0.20177 |
| Rwork | 0.197 |
| R-free | 0.29465 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3afm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.025 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.110 |
| Number of reflections | 7885 |
| <I/σ(I)> | 67.5 |
| Completeness [%] | 99.5 |
| Redundancy | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | PEG 4000, Lithium chloride, tris (hydroxymethyl) aminomethane |
