4W7G
Crystal Structure of the Dynein Light Intermediate Chain's Conserved Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.12 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 59.370, 138.670, 112.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.108 - 2.100 |
R-factor | 0.1766 |
Rwork | 0.174 |
R-free | 0.21960 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.232 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 1.350 | |
Number of reflections | 27518 | |
<I/σ(I)> | 12.3 | 1.7 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 7.3 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES, and 20% (w/v) PEG 10000 |