4W7F
Crystal Structure of Full-Length Split GFP Mutant E124H/K126H With Copper Mediated Crystal Contacts, C 2 2 21 Space Group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-28 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9789 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 68.200, 69.750, 82.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.760 - 2.900 |
R-factor | 0.271 |
Rwork | 0.264 |
R-free | 0.33200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3p |
RMSD bond length | 0.009 |
RMSD bond angle | 0.873 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: DEV_1555)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.760 |
High resolution limit [Å] | 2.900 |
Number of reflections | 4558 |
<I/σ(I)> | 7.98 |
Completeness [%] | 99.5 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 20% PEG8000, 0.1M CHES pH 9.5 |