4W7E
Crystal Structure of Full-Length Split GFP Mutant E124H/K126H With Copper Mediated Crystal Contacts, P 41 21 2 Space Group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-08 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 96.050, 96.050, 69.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.920 - 2.592 |
R-factor | 0.213 |
Rwork | 0.207 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3p |
RMSD bond length | 0.009 |
RMSD bond angle | 1.267 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: DEV_1555)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 67.920 |
High resolution limit [Å] | 2.592 |
Number of reflections | 10580 |
<I/σ(I)> | 19.81 |
Completeness [%] | 99.7 |
Redundancy | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1M Imidazole pH 8.0, 10% PEG8000 |