4W7D

Crystal Structure of Full-Length Split GFP Mutant D21H/K26H With Copper Mediated Crystal Contacts, P 21 21 21 Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	56.420, 82.050, 113.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.570 - 1.800
R-factor	0.183
Rwork	0.179
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.007
RMSD bond angle	1.321
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1555))

Data quality characteristics

	Overall
Low resolution limit [Å]	66.570
High resolution limit [Å]	1.799
Number of reflections	49634
<I/σ(I)>	8.8
Completeness [%]	99.6
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	298	20% PEG8000, 0.1M CHES pH9.5